High Pressure Liquid Chromatography

This unit discusses the separation of sample components by the process of high pressure liquid chromatography (HPLC). It explains the basic principles of the HPLC process and describes the major parts of a typical HPLC system, including a solvent pumping system, an autosampler, a column, a variable wavelength UV detector, and a data system. It describes the following major steps for performing an HPLC analysis: solvent preparation and delivery; sample preparation and injection; sample separation; component detection; and data processing. It also discusses how information presented on an HPLC chromatogram may be used for qualitative and quantitative analysis.



Duration: 0.8 hours